agarose gel


A Molecular Maze It Is

a: Agarose gel ~
b: complex maze with very tiny channels

What: "An agarose gel is like a complex maze with very tiny channels. DNA has a negative charge and will move toward the positive pole in an electrical field. Short pieces of DNA navigate more easily through the maze and therefore move a greater distance. An indicator dye is added to the DNA digest prior to injecting it into the gel so that the researcher can tell when the small fragments have run the length of the gel."

Writer: Jim Blankenship
Where: Reference Link Has Evaporated
Date: Aug 26 2013 5:47 PM

a: Agarose gel ~
b: strainer

What: "Proteins are like different sized noodles and the agarose gel is like a strainer, filled with little holes. Proteins that are small moves easily through the holes in the agarose gel, while larger proteins move more slowly, getting stuck in the holes in the gel. Thus, protein gel electrophoresis allows students to spread out the proteins from different bovine tissues, creating a protein fingerprint that serves as a barcode to identify that type of cell or tissue."

Writer: not stated
Date: Aug 26 2013 5:50 PM

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